Investigating Saporin-Conjugated Quantum Dots as a Microglia Depletion Strategy in the Substantia Nigra

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Creator: 

Landrigan, Jeffrey

Date: 

2017

Abstract: 

To study the relationship between neuroinflammation and neurodegeneration in Parkinson’s disease, we employed a relatively new biological assay using saporin-conjugated quantum dots. Conjugation of saporin, a type I ribosomal inhibitor protein, to quantum dots, which are fluorescent nanocrystals, generates a microglia-specific toxin (QD-SAP) which has been shown to selectively ablate microglia in vitro. Administration of QD-SAP in our studies shows the same microglial depletion observed in vitro, but in our in vivo studies, also shows neuronal loss. This effect in not localized to the SNc; administration of QD-SAP in the hippocampus (HPC) shows the same result. Interestingly, administration of QD-SAP in the HPC caused dopamine cell loss in the SNc. The reverse effect was not found. We suggest that it is an initial increase in inflammation, followed by a subsequent loss of homeostatic regulation upon microglial depletion, that causes neurons to die.

Subject: 

Neuroscience

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Neuroscience

Parent Collection: 

Theses and Dissertations

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