Development and Characterization of DNA Origami Nanostructures and their Application as an Aptamer-Mediated Targeted Delivery System

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Colquhoun, Fraser Charles Edward




The predictable geometries and intermolecular interactions of DNA have led to the development of many arbitrary nanoscale objects, including a class termed DNA origami. DNA origami nanostructure fabrication occurs through the folding of long, single-stranded DNA using short, single stranded oligonucleotides. Detailed herein is the development and characterization of DNA origami nanostructures with site-specific modifications for aptamer-mediated binding of target molecules. Molecular self-assembly is performed by thermal annealing and fabricated objects are purified by centrifugal filtration through molecular weight cut-off membranes. Nanostructures are characterized by agarose gel electrophoresis, UV-Vis quantification, and visualization by atomic force microscopy. Thrombin-binding aptamers, TBA15 and TBA29, were captured to the surface of rectangular DNA origami nanostructures and were incubated with the target, thrombin, to demonstrate the site-specific binding of target molecules. DNA origami nanostructures functionalized with aptamers for specific target recognition bear attractive potential for application in targeted therapies and targeted delivery of molecular payloads.


Health Sciences




Carleton University

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Theses and Dissertations

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