The Downstream Targets of MYB-Type Transcription Factors Involved in Suberin Biosynthesis

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.

Supplemental Files: 

Creator: 

Klein, Daniel Paul

Date: 

2019

Abstract: 

Suberin is a lipid- and phenolic-based heteropolymer that is deposited in the cell walls of certain plant tissues to act as a physical barrier against unregulated gas, water, or solute movement. Suberin deposition in root endodermis is regulated by MYB-type transcription factors, and under non-stress conditions these include AtMYB53, AtMYB92 and AtMYB93 in Arabidopsis. The transcriptional targets of these MYBs have not been previously reported. In this thesis, the transcriptomes of two Arabidopsis triple knockout myb53-myb92-myb93 mutants were compared to 14-day old wild-type seedlings using RNA-seq. Also, the transcriptome of a steroid-inducible AtMYB53 overexpression line was compared to an uninduced control in 10-day old seedlings. From these analyses, GDSL-type esterases / acyltransferases, an HXXXD-type (BAHD) acyl-transferase, additional MYB-type transcription factors, a WRKY transcription factor, ABCG-type transporters, and CASP-like assembly proteins were identified as suberin-related genes. Additionally, sequential C-terminal deletions of each of these MYBs revealed important regions for their activity.

Subject: 

Plant Physiology
Bioinformatics

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Biology

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).