Mass Spectrometry-based Anaylsis to Investigate the Pharmacokinetics and Proteomic Properties of a Viral Sensitizer

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.

Creator: 

Wistaff, Emma Anne

Date: 

2021

Abstract: 

Attenuated oncolytic viruses (OVs) are a promising alternative cancer therapy to mainstream methods such as radiotherapy and chemotherapy. OV therapy takes advantage of the defective antiviral response present in most cancer cells however heterogeneity amongst target cells and attenuation of OVs to increase their safety profiles has limited the efficacy of this treatment. We have developed novel small molecules named viral sensitizers (VSes) capable of enhancing viral infection and cancer-specific cell death. Liquid chromatography-mass spectrometry (LC-MS) methods were developed to study the pharmacokinetics (PK) and metabolomic activity of VSe1-28 via time course experiments. Parallel to this work, proteomic experiments were conducted to confirm the molecular target and mechanism of action of VSe1-28 through MRM and Auto-MS/MS methods. These new findings will aid in improving VSes and progress preclinical studies for clinical use in combination with OVs.

Subject: 

Chemistry

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Chemistry

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).