Fumonisin B1 Aptamer Optimization and Progress Towards Mycotoxin Nanoaptasensors

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.

Creator: 

Frost, Nadine

Date: 

2015

Abstract: 

Fumonisin B1 (FB1) and ochratoxin A (OTA) are agriculturally important mycotoxins that have implications in human and animal health. Aptamers are short single-stranded oligonucleotides that can be used as molecular recognition elements for the sensitive and selective detection of mycotoxins. Aptamers for FB1 and OTA were screened using a novel DNase I footprinting assay to characterize their structure and in- solution affinity. Two minimal binding aptamers for FB1 were identified that retain high binding affinity in solution and to a bound target. Progress was made towards fluorescence turn-on and turn-off sensors for FB1, utilizing semiconductor quantum dots and fluorescein in conjunction with metallic- and carbon-based quenchers. A fluorescence nano-aptasensor was developed as a simple paper test, with the capacity to detect OTA with a limit of detection between 10 – 100 nM. Rapid, robust aptasensors for FB1 and OTA have promising applications for in-field screening of mycotoxin residues on crops.

Subject: 

Chemistry
Biochemistry
Chemistry - Agricultural

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Chemistry

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).