Fumonisin B1 Aptamer Optimization and Progress Towards Mycotoxin Nanoaptasensors
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Fumonisin B1 (FB1) and ochratoxin A (OTA) are agriculturally important mycotoxins that have implications in human and animal health. Aptamers are short single-stranded oligonucleotides that can be used as molecular recognition elements for the sensitive and selective detection of mycotoxins. Aptamers for FB1 and OTA were screened using a novel DNase I footprinting assay to characterize their structure and in- solution affinity. Two minimal binding aptamers for FB1 were identified that retain high binding affinity in solution and to a bound target. Progress was made towards fluorescence turn-on and turn-off sensors for FB1, utilizing semiconductor quantum dots and fluorescein in conjunction with metallic- and carbon-based quenchers. A fluorescence nano-aptasensor was developed as a simple paper test, with the capacity to detect OTA with a limit of detection between 10 – 100 nM. Rapid, robust aptasensors for FB1 and OTA have promising applications for in-field screening of mycotoxin residues on crops.
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Copyright © 2015 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 2015
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frost-fumonisinb1aptameroptimizationandprogress.pdf | 2023-05-04 | Public | Download |