Methyl Epigenetic Mechanisms in the Freeze-Tolerance Response of Rana Sylvatica Nervous Tissue
Public Deposited- Resource Type
- Creator
- Abstract
Wood frog freeze tolerance is a classic example of metabolic rate depression (MRD), which facilitates reprioritization of minimal anaerobic resources to pro-survival pathways. Global gene expression is consequentially suppressed due, in part, to transcriptional controls, yet specific mechanisms have received little attention. Methylation of DNA and histone lysine residues are common epigenetic mechanisms associated with control of transcription and thus are implicated in MRD. However, preliminary findings appear tissue- and species-specific while research into nervous tissues is lacking. This thesis tracks the expression/activity of key methyl epigenetic modifiers and selected targets across the wood frog freeze-thaw cycle and associated sub-stresses. This thesis provides strong evidence for H3K9 and DNA hypomethylation during brain freeze recovery, largely correlated with expression of catalyzing enzymes. Non-histone roles are also suggested. Alleviation of repressive epigenetic controls likely contribute to a resumed permissive transcriptional state and may induce the activity of essential repair pathways during thawing.
- Subject
- Language
- Publisher
- Thesis Degree Level
- Thesis Degree Name
- Thesis Degree Discipline
- Identifier
- Rights Notes
Copyright © 2021 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
- Date Created
- 2021
Relations
- In Collection:
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
bloskie-methylepigeneticmechanismsinthefreezetolerance.pdf | 2023-05-05 | Public | Download |