To investigate the effect of ultrasound on the inulin from JA, JA powder, Purified JA inulin (PJAI) was treated with 20KHz ultrasound compared with chicory inulin (CI). Ultrasound treatment time had a positive linear relationship with reducing sugar content of these samples. Also, determined by HPLC and GPC, for PJAI, there was a negative linear relationship between high degree of polymerization (DP) inulin with ultrasound treatment time (R2=0.9169), and a positive linear relationship between low DP inulin with ultrasound treatment time (R2=0.9738), which was not observed for inulin from chicory. Furthermore, JA powder was added into whey and milk and treated with 20KHz ultrasound to investigate the structure change. Ultrasound changed the microstructure of milk resulting in a flatter surface for milk and whey. Ultrasound combined particles of milk and whey This kind of phenomenon was much more obvious only for milk when mixed with JA powder.