Investigation into the Mode of Antifungal Action of Berberine
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The motivation in this research is that Berberine’s antifungal mode of action is not known yet. The focus of this study is to identify the biochemical basis of yeast sensitivity to berberine (i.e. "mode of action"). The MIC of berberine with yeast was determined to be in the range of 0.156 to 0.078 mg/ml. To infer berberine mode of antifungal activity, GDA analysis was performed using a library of 4700 yeast strains, each with a different gene deletion, using a berberine concentration of 0.13 mg/ml. Out of the library, the 71 most sensitive yeast deletion mutant strains and 22 resistant strains were identified and analyzed with GO terms. Finally, using berberine’s natural florescence characteristic together with other histochemical stains, confocal fluorescence microscopy revealed that berberine was dynamically localized first to mitochondria and then to oil droplets within the yeast cell.
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Copyright © 2016 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 2016
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hashim-investigationintothemodeofantifungalaction.pdf | 2023-05-04 | Public | Download |