The mycelia from a culture of Fusarium avenaceum Fa120 (DAOM 196490) have been shown to produce enniatin A as a major product, along with smaller amounts of enniatins A1 and B1. In addition, this fungus was also found to produce two minor enniatins, enniatins B1-2 and A2, with enniatin A2 being the first N-methylleucine containing enniatin to be isolated from any Fusarium species. The crude fungal extract of Fusarium acuminatum MRC 3308 grown in SANDOZ medium was found to contain enniatins B, B1 and A1, with trace amounts of enniatin A. This is the first reported isolation of enniatins from this strain of F. acuminatum. In addition to the above enniatins, this strain also produces an N-methylleucine containing enniatin, enniatin B4, as well as an N-desmethyl enniatin, B2. The complete characterization of these new enniatins, as well as enniatins A1 and B1, which have never been fully characterized, is presented.