Biotransformation, Physico-Chemical Properties and Environmental Fate of Bisphenol A Bis(Diphenyl Phosphate): In Slico, In Vitro and Non-Target Metabolites using a Wister-Han Rat Liver Microsomal Model

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Creator: 

Herczegh, Sofia Mercedes

Date: 

2022

Abstract: 

Organophosphate ester (OPE) flame retardants and plasticizers have replaced several legacy, banned brominated flame retardants. Bisphenol A bis(diphenyl phosphate) (BPADP) exemplifies a growing industry trend towards production of complex, high molecular weight, 'novel' OPEs. An assay method was optimized for quantification of BPADP biotransformation to target metabolites bisphenol-A (BPA) and diphenyl phosphate (DPHP) in an in vitro Wistar-Han rat liver microsomal assay. In silico modelling via OECD Toolbox v4.4.1 and Non-Target Analysis (NTA) via Q-E-Orbitrap HRMS/MS were applied to predict physico-chemical properties and identify additional non-targeted metabolites of BPADP. DPHP and BPA were predicted in silico and confirmed in vitro, with BPADP demonstrating slow in vitro microsomal metabolism. Additional Phase I oxidation metabolites & one Phase II GSH adduct were identified via NTA. These findings add to the understanding of BPADP stability and biotransformation, factors highly applicable to hazard assessment of the compound as an alternative to legacy flame retardants.

Subject: 

Chemistry
Environmental Sciences
Toxicology

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Chemistry

Parent Collection: 

Theses and Dissertations

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