Interactions between temperature sensitive dnaB mutants of Escherichia coli and the plasmid R64-11
Public Deposited- Resource Type
- Creator
- Abstract
The observed suppression of the temperature sensitivity of chromosomal DNA synthesis in various dnaB mutants of Escherichia coli by a variety of large conjugative antibiotic. Resistance conferring plasmids (R factors) had been postulated to be due to the presence on these plasmids of a dnaB analog ( ban) gene. Attempts to support this hypothesis by physically locating and characterizing this postulated gene of the R factor R64-ll by means of~ vitro recombinant DNA and ON/\ hybridization techniques were unsuccessful. It was observed that no regions of this R factor DNA cloned into a multi-copy plasmid vector exhibited suppressing activity when genetically transformed into several dnaB temperature sensitive mutants , nor was there any detectable nucleotide sequence homology between the dnaB gene of E. coli and -- --R64-ll. Further investigation of this R factor- mediated suppresion phenomena provided data which tended not only to dispute the ban gene hypothesis; at least with respect to R64-ll, but to suggest that this suppression might be due to a generalized 'mutator' property of this plasmid.
- Subject
- Language
- Publisher
- Thesis Degree Level
- Thesis Degree Name
- Thesis Degree Discipline
- Identifier
- Access Rights
This work is available on request. You can request a copy at https://library.carleton.ca/forms/request-pdf-copy-thesis
- Rights Notes
Copyright © 1980 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
- Date Created
- 1980
Relations
- In Collection: