A multinuclear NMR study of the binding of cis-[Pt(NH[subscript 3]) [subscript 2]Cl[subscript 2]] and trans-[Pt(NH[subscript 3]) [subscript 2]Cl[subscript 2]] with methylated nucleosides : synthesis and stereochemistry
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A multinuclear NMR approach has been taken to study the metal ion binding sites of cis-[Pt(NH3)2Cl2] and trans-[Pt(NH3)2Cl2], to several methylated nucleosides. 1H and 13C NMR provide an indirect method of monitoring the platination site, whereas 15N NMR provides a more direct probe. Mass spectroscopy (FAB) was employed to deterrnine if the metal ion-complex was a mono or bis methyl nucleoside adduct. All of the purine and pyrimidine bases formed bis adducts with the exception of the reaction between cis-[Pt(NH3)2Cl2] and 1-methyladenosine, which formed a mono adduct.The cis-[Pt(NH3)2Cl2] and the trans-[Pt(NH3)2Cl2] complexes were found to bind the N-7 position of 1-methylguanosine. The 7N-methylguanosine did not react with either the cis- or trans-[Pt(NH3)2Cl2]. Cis-[Pt(NH3)2(NO3)2] and trans-[Pt(NH3)2(NO3)2] were found to bind the N-1 position of 3-methyluracil and 3-methylcytosine.
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Copyright © 1987 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 1987
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