Pharmacological Inhibition of PTEN, using BpV (Phen), Promotes Survival of Retinal Ganglion Cells In Vitro and In Vivo

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Previous work has shown that potassium bisperoxo (1,10-phenanthroline) oxovanadate, BpV(Phen), is a potent protein phosphotyrosine phosphatase (PTPase) inhibitor that preferentially blocks the activity of Phosphatase Tensin Homologue Deleted on Chromosome Ten (PTEN). Currently, it remains unknown how pharmacological inhibition of PTEN by bpV(phen) impacts the survival of mammalian RGCs. Thus, the current study investigated the effect of bpV(phen) on RGC survivalin vitroandin vivoand the mechanism mediating this outcome following bpV(phen) administration. Collectively, this study revealed that bpV(phen) promoted survival of injured retinal ganglion cells from mature animals (postnatal day 21 (P21) and adult mice) after injuryin vitroandin vivo. Interestingly, data also showed bpV(phen) to have an antagonistic effect on healthy RGCs and promote apoptosis. Lastly, preventing Smad3 from being phosphorylated in the transforming growth factor β (TGF-β) pathway did not augment the survival in bpV(phen)-treated RGCs.

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Copyright © 2019 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.

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