Macrophytes impact the structure and function of freshwater ecosystems but can also have an adverse impact on human activity. While the efficacy of macrophyte control methods are well-known, there are few studies on the environmental impacts of these treatments. In a pair of studies, the impacts of both mechanical cutting and the application of benthic barriers used in the control of the invasive Myriophyllum spicatum on the physical, chemical and biological components of four Ontario Lakes were investigated over four months. A Before-After-Control-Impact study design was employed, and three of the lakes were selected across a nutrient gradient. Observed impacts were minimal and not consistent across lakes. Nevertheless, some significant changes were observed for temperature, pH and zooplankton density. Changes to benthic macroinvertebrate and zooplankton community structure were observed in all four lakes. There were no significant differences in control-impact nutrient levels in any of the lakes.