Selection of DNA Aptamers for Mycotoxins

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.


Ruscito, Annamaria




Mycotoxins deoxynivalenol (DON) and patulin (PAT) are toxic fungal metabolites that contaminate food commodities worldwide. They pose health risks for humans and animals, and have a negative impact on the economy. Mycotoxin detection is vital to ensure public safety. Although traditional detection techniques for mycotoxin detection (e.g. LC-MS) have proven to be highly selective and sensitive, simpler, more rapid, and more cost-effective approaches are required. Aptamer-based techniques are a possible solution. Aptamers are oligonucleotides that can bind to molecules with high affinity and specificity, generated via an in vitro process known as SELEX. We attempted to generate aptamers for DON and PAT. The selection process for DON was challenging and it would be beneficial to continue SELEX, whereas the process for PAT was simpler thus sequences for potential aptamers were obtained. This work has contributed to the production of aptamer-based assays and biosensors for mycotoxin detection.




Carleton University

Thesis Degree Name: 

Master of Science: 

Thesis Degree Level: 


Thesis Degree Discipline: 

Chemical and Environmental Toxicology

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).