Fibroblast growth factor 2 (FGF2) is exclusively expressed by astrocytes and has hypophagic and weight reducing effects. The objective of this study is to further elucidate the role of FGF2 on feeding and energy balance using FGF2 KO animals. Mice were exposed to regular chow or a 60% high fat diet over the course of 6 weeks. We hypothesized FGF2KO animals would exhibit hyperphagia and weight gain, and that this would augment in the presence of a high fat diet. Results demonstrated both WT and FGF2KO animals gained weight similarly and had similar caloric intake when ingesting high fat diet. Carbohydrate expenditure was increased in male KO's and female KO's had increased ambulatory counts during the night phase. Future studies should investigate the effects of stressed FGF2KO animals on HFD and should continue to explore the circuitry involved in energy balance and regulation.