Investigation of factors influencing the d-Aminolevulinic acid dehydratase assay of blood-lead with a specific reference to the in vitro effects of monovalent cations
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Investigation of factors influencing the δ-aminolevulinic acid dehydratase (ALAD) for monitoring lead-blood concentration was performed with a view towards elimination of procedural artifacts. Using the optimum procedure the in vitro effects of the monovalent cations of Li, Na, K, Cs, NH4, Tl, Hg, and Ag on the pH-activity profile of ALAD from human blood over a seven day period from the date of blood acquisition were investigated and displayed. Several interferences, most notably Hg(II) from HgCl2, were identified and it was found that Cu(I) and Ag(I) deactivated the enzyme while others showed negligible effects on the pH-activity profile and especially on a predefined pH-activity ratio of activities at pH values 6.3 and 7.2 .
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This work is available on request. You can request a copy at https://library.carleton.ca/forms/request-pdf-copy-thesis
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Copyright © 1981 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 1981
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