Less is MTOR: Regulation of Protein Synthesis via the Insulin Signaling Pathway in the Anoxia-Tolerant Red-Eared Slider Trachemys Scripta Elegans

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.


Szereszewski, Kama




The red-eared slider turtle, can survive 3-4 months of anoxic submergence in cold water during the winter. The effect of hypoxia/anoxia on protein synthesis in the turtle was investigated with a focus on the insulin-signaling pathway and analysis of the mammalian target of rapamycin (mTOR) and its upstream and downstream effectors in liver and white muscle. Expression of mTORC1 did not change in muscle but increased significantly in liver after 5 and 20 hours of anoxic submergence. Upstream effectors, AKT and RAPTOR, were also elevated in liver but suppressed in muscle. PRAS40 and TSC2
inhibitors of mTOR were differentially regulated in both tissues but generally suppressed. Downstream targets of mTOR signaling (eIF4E, 4E-BP1, P70S6K, S6) as well as the poly(A) binding protein also showed differential responses to anoxia. Overall, the data indicate that the early response to anoxia is maintenance of protein synthesis in liver but suppression in white muscle.


Biology - Molecular




Carleton University

Thesis Degree Name: 

Master of Science: 

Thesis Degree Level: 


Thesis Degree Discipline: 


Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).