Antioxidant and Calcium Binding Activities of Hydrolyzed Oat Bran Proteins and Chromatographic Fractions

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.

Creator: 

Baakdah, Morooj

Date: 

2015

Abstract: 

Interest in peptides as bioactive agents has gained attention. Oats are a source of dietary fibers and phenols with demonstrated health benefits. However peptides have received little attention. In this research, oat bran treated with viscozyme and digested with protamex. The hydrolysates separated on RP-HPLC to eight fractions. The fractions were assayed for their ability to scavenge radicals, chelate metals, inhibit peroxide and bind calcium. In ORAC assay, F7 had the highest peroxyl quenching activity compared to OPH and other fractions. In the superoxide radical assay F3 and F6-F8 had
higher activities while in the hydroxyl radical assay, F4, F7, F8 were the highest (14-16%).In the metal chelation assay F1 and OPH had the best iron chelating in comparison to the RP-HPLC derived peptides (1.22–22.8%). The calcium binding activity of OPH and peptides F5 and F7 was weak. Spectrums/MS charged peaks in F5 and F7 revealed a number peptides.

Subject: 

Food Science and Technology
Nutrition
Food Science and Technology

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Chemistry

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).