Characterization and Application of a Histamine Aptamer-Based Biosensor

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Creator: 

Iraganje, Elysee

Date: 

2021

Abstract: 

Histamine is a biogenic amine, identified as a natural contaminant in food and alcoholic beverages, with high levels eliciting allergy-related conditions. Exposure to allergens can lead to abnormal high levels of histamine in the body that is detectable in blood and urine. A rapid histamine biosensor that can detect histamine levels in food, beverages and also be used as a diagnostic tool for allergy-related conditions will be an ideal point of care testing tool. In addition, histamine has been identified as a close-range aggregation pheromone component in bed bug feces. A portable biosensor that detects bed bugs in these settings is in demand. Nano-aptamer based biosensors have previously been used to detect wide range of targets. In this study, histamine-binding aptamers were characterized. A head-to-head comparison of binding affinity and specificity for these group of aptamers were conducted, and the aptamer candidates best suited for biosensor application was selected.

Subject: 

Biochemistry
Analytical Chemistry
Inorganic Chemistry

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Chemistry

Parent Collection: 

Theses and Dissertations

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