Novel MOS-Based Biomolecule Detection Techniques

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.


Griffin, Ryan Hunter




This thesis advances the state of the art in biomolecule detection by allowing for quantitative detection using radioactive labels whose decay is detected by a pixelated complementary metal-oxide-semiconductor (CMOS), or CMOS-compatible, sensor. Additionally, the usefulness of this technology as a testbed for radioimmunotherapy (RIT) pharmaceuticals is considered. For the first time a CMOS image sensor has been used to detect the presence of radiolabelled target biomolecules captured on a functionalized surface. Using aptamer functionalization the system successfully detected phosphorus-32 labelled adenosine triphosphate (ATP) at a surface concentration of 2.3 x 10^7 molecules/cm^2, well below those typically associated with fluorescence-based sensor architectures. The system has also demonstrated its amenability to multiplexed biomolecule detection. Geant4, a Monte Carlo toolkit for simulating the passage of radiation through matter, was used to model the detection system. This system has applications in quantitative biomolecule detection and in the development of RIT pharmaceuticals employing beta particle emitting isotopes. Also for the first time, a MOS sensor has been designed, fabricated, and tested for use in the characterization of targeted alpha therapy (TAT) pharmaceuticals. The sensor consists of a 16 by 16 array of 100 micrometer square alpha particle sensitive cells fabricated in-house using a simple nMOS process. A subset of the cells are functionalized for the attachment of chelators under investigation for new pharmaceuticals. To demonstrate the utility of this sensor as a characterization platform, cells functionalized with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-DNA conjugates were used to chelate americium-241 from solution, and the alpha particle emissions over the surface of the IC measured. The IC was able to quantitatively determine the amount of alpha emitter present over each cell, allowing the chelator and chelating chemistry to be assessed. Without any optimization of the chelation chemistry, a 21% increase of emissions was detected on cells functionalized with DOTA relative to unfunctionalized cells.


Engineering - Electronics and Electrical
Physics - Radiation
Biophysics - Medical




Carleton University

Thesis Degree Name: 

Doctor of Philosophy: 

Thesis Degree Level: 


Thesis Degree Discipline: 

Engineering, Electrical and Computer

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).