Assessment of Viral Vaccine N-Glycosylation via Orthogonal Capillary Electrophoresis-Based Methods
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Despite global vaccination programs, seasonal influenza continues to pose a significant economic burden and strain on healthcare systems worldwide. Annual reformulation of influenza vaccines is required due to the high mutation rates of surface glycoproteins on influenza viruses. Although vaccination is the most effective preventative measure against influenza viruses, reduced vaccine efficacy in recent years has become an increasing concern. Egg-adaptations incurred through the manufacturing process of influenza vaccines result in antigenic mismatches, whereby vaccine strains differ from the wild-type circulating viruses. In 2017, low vaccine efficacy was attributed to altered glycosylation characteristics of the H3N2 vaccine strain whereby an egg-adaptation resulted in the deletion of a single N-glycosylation site. However, even with these implications on immunogenicity, influenza glycosylation is not a well-implemented critical quality attribute and therefore not optimally controlled or regulated during vaccine manufacturing due to assay limitations.
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Copyright © 2022 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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bigelow-assessmentofviralvaccinenglycosylationvia.pdf | 2023-05-05 | Public | Download |