The Effect of Fasting on LH Secretion in Female Rats with Mutations to the Ghrelin Receptor Gene
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We investigated how ghrelin can mediate the inhibitory effects of fasting on the reproductive axis using female rats with a point mutation to the gene encoding GHSR. For this purpose, we fasted FHH GHSR KO rats and their WT counterparts starting on the evening of metestrus and ending 42h later on the expected proestrus afternoon. Rats were then euthanized; blood was collected and analyzed to compare plasma LH and estrogen concentrations between these animals and controls. Fasting significantly decreased LH concentrations in all rats regardless of their genotype. Interestingly, GHSR KO rats showed overall higher LH concentrations than WT rats regardless of their condition. Estrogen levels were not influenced by the genetic mutation and fasting. These findings suggest that ghrelin signalling is not required for a fasting-induced decrease in LH concentrations. However, ghrelin may modulate reproductive function as a negative feedback signal that reduces LH release regardless of energy state.
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Copyright © 2014 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 2014
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