Using RNA-Seq to identify oospore wall-specific Carbohydrate-Active Enzyme (CAZyme) coding genes of Pythium ultimum var. ultimum, an oomycete plant pathogen

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat or click here to download the PDF file.

Click here to download the PDF file.

Supplemental Files: 

Creator: 

Giroux, Emily

Date: 

2017

Abstract: 

Oospores are propagules resulting from oomycete sexual recombination and act as long-term survival structures. In Pythium ultimum, and many other oomycete species, the glucan-rich walls of dormant oospores can account for 50% of the total volume of the oospore. Oospore walls can be digested quickly and used as a food source to propel oospores towards a germinable state. Whether digestion occurs by enzymes resulting from genes transcribed at this stage, or prepackaged transcripts/proenzymes released upon wall rehydration is currently unknown. Since oospore walls are composed predominantly of β-glucans, enzymatic digestion by glucanases may represent the earliest stage-specific molecular development ocurring at this point in its life cycle. We used RNA-Seq to investigate the oospore conversion process and to identify genes encoding oospore wall-specific enzyme products. Some enzymes were expressed solely during oospore conversion, and among those, some are only present in Pythium species when compared to other oomycetes.

Subject: 

Biology - Molecular
Bioinformatics
Genetics

Language: 

English

Publisher: 

Carleton University

Thesis Degree Name: 

Master of Science: 
M.Sc.

Thesis Degree Level: 

Master's

Thesis Degree Discipline: 

Biology

Parent Collection: 

Theses and Dissertations

Items in CURVE are protected by copyright, with all rights reserved, unless otherwise indicated. They are made available with permission from the author(s).