New Derivatization Strategies for Installation of Fixed, Permanent Charges onto Analytes of Biological and Environmental Concern
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Mass spectrometry (MS) is a highly sensitive analytical technique that can be used to identify thousands of analytes from a single biological matrix. Chemical derivatization (CD) reagents can enhance analyte detection and quantification by adding a specific functional group that enhances ionization efficiency and stability. The goal of this project is to design a new CD reagent by synthesizing a Meldrum's acid derivative containing a fixed permanent charge (MAD+) to be used as a stable ketene precursor. Synthesis of a MAD+ was explored via alkylation and Knoevenagel condensation reactions but proved unfruitful. However, C-alkylation with 6-bromohexanoic acid via carbodiimide coupling, reduction and amine alkylation produced a MAD+ in generous yields. The reaction of this MAD+ with 3,5-di-tert-butylphenol demonstrated its ability to induce its fixed permanent charge onto analytes, allowing its detection in MS. Purification of the final MAD+ remained an issue and should be investigated and optimized in future works.
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Copyright © 2022 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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- 2022
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aulenback-newderivatizationstrategiesforinstallation.pdf | 2023-05-05 | Public | Download |